Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26529||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerAddgene plasmid 12282
- Backbone size w/o insert (bp) 6500
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Alt nameBIC (part of Exon3)
SpeciesH. sapiens (human)
Insert Size (bp)500
Entrez GeneMIR155 (a.k.a. MIRN155, miRNA155, mir-155)
/ Fusion Protein
- eGFP (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pLXSN-5
- 3′ sequencing primer MSCV-rev (Common Sequencing Primers)
Terms and Licenses
Articles Citing this Plasmid
MG155 was derived originally from pMIG-w. The GFP and IRES were removed using BglII and HindIII, and a new cassette with eGFP (about 700 bps) followed by a miR-155 sequence (about 500 bps with flanking Not1 and XhoI sites) was cloned in using BamHI (destroying the BglII site) and HindIII. This results in a 7 kb plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MG-155 was a gift from David Baltimore (Addgene plasmid # 26529 ; http://n2t.net/addgene:26529 ; RRID:Addgene_26529)
For your References section:Sustained expression of microRNA-155 in hematopoietic stem cells causes a myeloproliferative disorder. O'Connell RM, Rao DS, Chaudhuri AA, Boldin MP, Taganov KD, Nicoll J, Paquette RL, Baltimore D. J Exp Med. 2008 Mar 17. 205(3):585-94. 10.1084/jem.20072108 PubMed 18299402