MSCV puro let-7 sponge
Vector backboneMSCV puro
- Backbone size w/o insert (bp) 6000
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Full plasmid sequence is available only if provided by the depositing laboratory.
Gene/Insert namelet-7 sponge
Insert Size (bp)150
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site blunt (unknown if destroyed)
- 5′ sequencing primer pLXSN-5 (Common Sequencing Primers)
The let-7 family sponge contains six bulged sites with alternating sequences AACUAUACAAGGACUACCUCA and AACUAUACAAUGACUACCUCA. The binding sites are for a consensus sequence of all mammalian let-7 miRNAs
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MSCV puro let-7 sponge was a gift from Phil Sharp (Addgene plasmid # 29766)
For your References section:Suppression of non-small cell lung tumor development by the let-7 microRNA family. Kumar MS, Erkeland SJ, Pester RE, Chen CY, Ebert MS, Sharp PA, Jacks T. Proc Natl Acad Sci U S A. 2008 Feb 28. ():. 10.1073/pnas.0712321105 PubMed 18308936
Map generated by Addgene from plasmid data.