pLenti CMV/TO eGFP Puro (w159-1)
(Plasmid #17481)

• Purpose: 3rd gen lentiviral eGFP expression vector, CMV/TO promoter, Puro 
• Depositing Labs: Eric Campeau, Paul Kaufman
• Publication: Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Backbone

• Vector backbone: p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
• Backbone size w/o insert (bp): 8052
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stbl3, 37oC
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: enhanced green fluorescent protein
• Alt name: GFP
• Insert Size (bp): 719

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMVforw

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• A portion of this plasmid was derived from a plasmid made by: eGFP gene is derived from peGFP-C1 plasmid (Clontech).
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the full sequence was assembled and may therefore contain sequence discrepancies within the backbone that do not affect plasmid function.