pET-29b(+)-mSandy2
(Plasmid #177760)

• Purpose: Expresses mSandy2 using the T7 Promoter
• Depositing Lab: Roberto Chica
• Publication: Legault et al Chem Sci. 2022 Jan 11;13(5):1408-1418. doi: 10.1039/d1sc05088e. eCollection 2022 Feb 2.

Backbone

• Vector backbone: pET-29b(+)
• Backbone manufacturer: Twist Bioscience
• Backbone size w/o insert (bp): 5234
• Total vector size (bp): 5969
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mSandy2
• Species: Synthetic
• Insert Size (bp): 735
• Promoter: T7 Promoter
• Tag / Fusion Protein:
  • 6xHis tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: TAA TAC GAC TCA CTA TAG GG
• 3′ sequencing primer: GCT AGT TAT TGC TCA GCG G

Resource Information

• Supplemental Documents:
  • mSandy2.gb
• A portion of this plasmid was derived from a plasmid made by: Twist Bioscience

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry