CJ52 (cg1849 utr mut)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17780||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepMT-puro with renilla luciferase
Backbone manufacturerpMT-puro from David Sabatini Lab
Vector typeInsect Expression, Luciferase ; Drosophila metallothionein gene promoter drives expression
Growth in Bacteria
Gene/Insert namecg1849 utr fragment mut
SpeciesD. melanogaster (fly)
Insert Size (bp)450
/ Fusion Protein
- renilla luciferase (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer MT forward (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CJ52 (cg1849 utr mut) was a gift from David Bartel (Addgene plasmid # 17780 ; http://n2t.net/addgene:17780 ; RRID:Addgene_17780)
For your References section:Intronic microRNA precursors that bypass Drosha processing. Ruby JG, Jan CH, Bartel DP. Nature. 2007 Jul 5. 448(7149):83-6. 10.1038/nature05983 PubMed 17589500
Map uploaded by the depositor.