pPro18
(Plasmid #17806)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pPro18
  • Backbone size (bp) 5135
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

The PCR product containing prpR and PprpB was digested with ClaI and NheI and ligated to the large fragments of pBAD18 resulting from digestion with the same enzymes, creating pPro18.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPro18 was a gift from Jay Keasling (Addgene plasmid # 17806)
  • For your References section:

    A propionate-inducible expression system for enteric bacteria. Lee SK, Keasling JD. Appl Environ Microbiol. 2005 Nov . 71(11):6856-62. 10.1128/AEM.71.11.6856-6862.2005 PubMed 16269719