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Plasmid 17806: pPro18
  • None

  • pPro18
    (Search Vector Database)

  • Bacterial Expression, Synthetic Biology

  • 5135

  • n/a List of Sequencing Primers

  • pBAD Reverse

  • Ampicillin

  • DH10B

  • 37

  • High Copy

  • View sequences (1)
  • View map

  • Jay Keasling

  • UBMTA

Comments: 

The PCR product containing prpR and PprpB was digested with ClaI and NheI and ligated to the large fragments of pBAD18 resulting from digestion with the same enzymes, creating pPro18.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: A propionate-inducible expression system for enteric bacteria. Lee et al (Appl Environ Microbiol. 2005 Nov . 71(11):6856-62. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17806" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only