|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17810||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5878
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
Copy numberLow Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a
- 3′ sequencing primer pBAD reverse (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
After deletion of the NheI site in the front of pACYC origin region of pBAD33 by partial digestion with NheI, T4 DNA polymerase treatment, and self-ligation, the ClaI-NheI prpR-PprpB region from pPro18 was ligated to the large fragments of pBAD33 resulting from digestion with the same enzymes, creating pPro33.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pPro33 was a gift from Jay Keasling (Addgene plasmid # 17810 ; http://n2t.net/addgene:17810 ; RRID:Addgene_17810)
For your References section:A propionate-inducible expression system for enteric bacteria. Lee SK, Keasling JD. Appl Environ Microbiol. 2005 Nov . 71(11):6856-62. 10.1128/AEM.71.11.6856-6862.2005 PubMed 16269719