Addgene - pPro30 Plasmid Data

Plasmid 17809: pPro30

Gene/insert name:
None
Vector backbone:
pPro30
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size (bp):
5445
5' sequencing primer:
n/a List of Sequencing Primers
3' sequencing primer:
pBAD reverse
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH10B
Growth temperature (℃):
37
High or low copy:
Low Copy
Sequence: View sequences (1)
Principal Investigator:
Jay Keasling
Terms and Licenses:
MTA

Comments:

After deletion of the NheI site in the front of pACYC origin region of pBAD30 by partial digestion with NheI, T4 DNA polymerase treatment, and self-ligation, the ClaI-NheI prpR-PprpB region from pPro18 was ligated to the large fragments of pBAD30 resulting from digestion with the same enzymes, creating pPro30.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A propionate-inducible expression system for enteric bacteria. Lee et al (Appl Environ Microbiol. 2005 Nov . 71(11):6856-62. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17809" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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