|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17861||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2900
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameFLIPrbs T135A
Alt nameRibose Binding Protein
Insert Size (bp)2400
/ Fusion Proteins
- ECFP (N terminal on insert)
- EYFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
T135A was generated using QuikChange (Stratagene) in the mutant background of FLIPrib-250n.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:FLIPrbs T135A was a gift from Wolf Frommer (Addgene plasmid # 17861 ; http://n2t.net/addgene:17861 ; RRID:Addgene_17861)
For your References section:Development of a fluorescent nanosensor for ribose. Lager I, Fehr M, Frommer WB, Lalonde S. FEBS Lett. 2003 Oct 9. 553(1-2):85-9. 10.1016/S0014-5793(03)00976-1 PubMed 14550551