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FLIPrbs
(Plasmid #17860)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17860 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRSET-B
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLIPrbs
  • Alt name
    FLIPrib-250n
  • Alt name
    ECFP-RBP-EYFP
  • Alt name
    Ribose Binding Protein
  • Species
    E. coli
  • Insert Size (bp)
    2400
  • Mutation
    D208G
  • Tags / Fusion Proteins
    • ECFP (N terminal on insert)
    • EYFP (C terminal on insert)

Cloning Information

Resource Information

Depositor Comments

A RBP PCR product from genomic E. coli DNA encoding mature RBP was cloned into the KpnI site of FLIPglu-600u in pRSETB, replacing the glucose sensor's mglB moiety. The RBP sequence was found to carry a D208G substitution, which does not significantly affect the binding affinity.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLIPrbs was a gift from Wolf Frommer (Addgene plasmid # 17860 ; http://n2t.net/addgene:17860 ; RRID:Addgene_17860)
  • For your References section:

    Development of a fluorescent nanosensor for ribose. Lager I, Fehr M, Frommer WB, Lalonde S. FEBS Lett. 2003 Oct 9. 553(1-2):85-9. 10.1016/S0014-5793(03)00976-1 PubMed 14550551