Addgene - pMXs-hKLF4 (Plath) Plasmid Data

Gene/insert name:
Kruppel-like factor 4 (gut)
Alt name:
KLF4
Insert size:
1413
Species:
H. sapiens (human)
Entrez Gene:
KLF4 (RP11-150J11.1, EZF, GKLF)
Mutation:
N/A
Vector backbone:
pMXs
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert:
4622
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
Not I
Site destroyed during cloning:
No
5' sequencing primer:
cctctagactgccggatctagcta List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers, other:
none
Person or lab that originally
cloned the gene/insert:
pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H.
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Kathrin Plath
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Generation of human induced pluripotent stem cells from dermal fibroblasts. Lowry et al (Proc Natl Acad Sci U S A. 2008 Feb 26. 105(8):2883-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17967" in your Materials and Methods section.