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CMV-HA-hTAF1
(Plasmid #17997)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17997 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hTAF1
  • Alt name
    hTAFII250
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6000
  • Entrez Gene
    TAF1 (a.k.a. BA2R, CCG1, CCGS, DYT3, DYT3/TAF1, KAT4, MRXS33, N-TAF1, NSCL2, OF, P250, TAF(II)250, TAF2A, TAFII-250, TAFII250, XDP)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site StuI (destroyed during cloning)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Depositor Comments

See author's map for more information. A BamHI/KpnI fragment from pHAX250 (pCMVHAX hTAFII250) was cloned into BamHI/StuI cut pCS2+.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV-HA-hTAF1 was a gift from Robert Tjian (Addgene plasmid # 17997 ; http://n2t.net/addgene:17997 ; RRID:Addgene_17997)
  • For your References section:

    Cloning and expression of human TAFII250: a TBP-associated factor implicated in cell-cycle regulation. Ruppert S, Wang EH, Tjian R. Nature. 1993 Mar 11. 362(6416):175-9. 10.1038/362175a0 PubMed 7680771