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Plasmid 18121: pHIV-Zsgreen
  • Zsgreen

  • Zoanthus sp. green fluorescent protein

  • 700

  • Anthozoa

  • AF168422

  • pSICO
    (Search Vector Database)

  • Mammalian Expression, Lentiviral

  • 6900

  • MscI

  • No

  • ClaI

  • No

  • n/a List of Sequencing Primers

  • Ampicillin

  • Stbl3

  • 37

  • High Copy

  • Zsgreen is from Clontech and pSICO is from Tyler Jacks.

  • View sequences (2)
  • View map

  • Bryan Welm

  • Zena Werb

  • UBMTA
    Clontech Limited Use Label License

Comments: 

HIV-Zsgreen was cloned by removing the U6-TATAlox-CMVie-EGFP-TATAlox- WPRE content of pSICO (Ventura et al., 2004), and adding the EF1-alpha promoter, a multiple cloning site (MCS), an internal ribosome entry site (IRES) and Zsgreen. cDNA can be cloned into the MCS (NotI, EcoRI, HpaI, XbaI, SmaI, and BamHI sites) to enable bicistronic expression with Zsgreen.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Lentiviral transduction of mammary stem cells for analysis of gene function during development and cancer. Welm et al (Cell Stem Cell. 2008 Jan 10. 2(1):90-102. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18121" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only