|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18121||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6900
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Copy numberHigh Copy
Alt nameZoanthus sp. green fluorescent protein
Insert Size (bp)700
- Cloning method Restriction Enzyme
- 5′ cloning site MscI (not destroyed)
- 3′ cloning site ClaI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
HIV-Zsgreen was cloned by removing the U6-TATAlox-CMVie-EGFP-TATAlox- WPRE content of pSICO (Ventura et al., 2004), and adding the EF1-alpha promoter, a multiple cloning site (MCS), an internal ribosome entry site (IRES) and Zsgreen. cDNA can be cloned into the MCS (NotI, EcoRI, HpaI, XbaI, SmaI, and BamHI sites) to enable bicistronic expression with Zsgreen.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pHIV-Zsgreen was a gift from Bryan Welm & Zena Werb (Addgene plasmid # 18121)
For your References section:Lentiviral transduction of mammary stem cells for analysis of gene function during development and cancer. Welm BE, Dijkgraaf GJ, Bledau AS, Welm AL, Werb Z. Cell Stem Cell. 2008 Jan 10. 2(1):90-102. 10.1016/j.stem.2007.10.002 PubMed 18371425