miR-124
(Plasmid #182363)

• Purpose: Plasmid with 4x miR-124 target sites
• Depositing Lab: Mario Capecchi
• Publication: Additional gene targeting vector construction plasmids (unpublished)

Backbone

• Vector backbone: Custom
• Backbone manufacturer: Hyung-song Nam, Capecchi laboratory
• Vector type: Mammalian Expression, Cre/Lox, Synthetic Biology
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 4x miR-124 target sites
• Species: Synthetic

Resource Information

• Supplemental Documents:
  • ** CAG-LSL-miR-124-bpA.gb
• A portion of this plasmid was derived from a plasmid made by: CAG promoter is from pCAGGS obtained from BCCM/LMBP. Neo* is from Addgene plasmid #22687. bGH pA is from Neal Copeland. SV40 pA is from Clontech. 4x miR-124 target site sequence was synthesized from oligonucleotides.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Four copies of miR-124 target sites (Åkerblom et al., 2012, https://doi.org/10.1523/JNEUROSCI.0558-12.2012) were cloned into “StLa” reporter plasmid (pCAG-loxP-pEM7-Neo*-bGHpA-3xSV40pA-loxP-tauLacZ-bGHpA from Nam and Benezra, 2009, https://doi.org/10.1016/j.stem.2009.08.017) without the tauLacZ insert and the Rosa26 homology arms. The 4x miR-124 target sequence can be released with BamHI and NotI.

More information on the “StLa” reporter at
(1) http://www.informatics.jax.org/allele/MGI:4366911
(2) https://figshare.com/ndownloader/files/38884434 (clicking this link will download a file)