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(Plasmid #1833)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 1833 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
    Mammalian Expression, Retroviral, Cre/Lox
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    retroviral RET construct
  • Alt name
    removable exon trap
  • Alt name
    short (weak) RNA pol II promoter for NEO expression

Cloning Information

Resource Information

Depositor Comments

See scanned map.
This is the original RET-EGFP vector. The highest virus titer that can be obtained with this construct is ~4x10^4 cfu/ml: the mRNA instability signal seems to block transcription of the virus genome at least to some extent. There are two versions, N1 and N2. Version N1 contains two XbaI sites and can be used for transfection after isolation of the XbaI-XbaI fragment. However, N1 contains an additional cryptic poly A signal between EGFP and GHpA, and its orientation is the same as that of virus transcription (from right to left in the figure). This means that N1 can not be used for the virus production: a full-length virus RNA is not produced efficiently. In contrast, N2 does not have such a cryptic poly A signal and is suitable for virus production. However, N2 contains an additional XbaI site between EGFP and GHpA, making it very difficult to isolate all the essential components of the vector on a single XbaI-XbaI fragment. Because of a weak NEO promoter, the number of G418-resistant colonies obtained after transfection (N1) or infection (N2) is fewer than that generated by other RET vectors with a strong NEO promoter.
(Leder #C-1010)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRET.IS.IRES-EGFP N1 was a gift from Philip Leder (Addgene plasmid # 1833)
  • For your References section:

    RET: a poly A-trap retrovirus vector for reversible disruption and expression monitoring of genes in living cells. Ishida Y, Leder P. Nucleic Acids Res 1999 Dec 15;27(24):e35. 10.1093/nar/27.24.e35 PubMed 10572187