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Rictor_2 shRNA
(Plasmid #1854)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 1854 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLKO.1
  • Backbone manufacturer
    Stewart SA, RNA 2003 Apr; 9(4):493-501.
  • Backbone size w/o insert (bp) 7032
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Unknown
  • Growth instructions
    XL10-Gold Ultracompetent Cells from Stratagene. 37oC.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rictor
  • Alt name
    shRNA Rictor
  • Alt name
    333B Rictor_2
  • gRNA/shRNA sequence
    5'-CGGGCAGCCTTGAACTGTTTAACTTCCTGTCATTAAACAGTTCAAGGCTGCTTTTTGAATT-3'
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    60
  • GenBank ID
    NM_152756
  • Entrez Gene
    RICTOR (a.k.a. AVO3, PIA, hAVO3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer LKO.1 5'
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This plasmid is referred to as Rictor_1 in the science paper. Sense: 5

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Rictor_2 shRNA was a gift from David Sabatini (Addgene plasmid # 1854 ; http://n2t.net/addgene:1854 ; RRID:Addgene_1854)
  • For your References section:

    Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Sarbassov DD, Guertin DA, Ali SM, Sabatini DM. Science 2005 Feb 18;307(5712):1098-101. 10.1126/science.1106148 PubMed 15718470