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Raptor_1 shRNA
(Plasmid #1857)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pLKO.1
  • Backbone manufacturer
    Stewart SA, RNA 2003 Apr; 9(4):493-501.
  • Backbone size w/o insert (bp) 7032
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Unknown
  • Growth instructions
    XL10-Gold Ultracompetent Cells from Stratagene. 37oC.
  • Copy number
    Unknown

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    Raptor
  • Alt name
    65 Raptor_1
  • Alt name
    shRNA Raptor
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    60
  • Entrez Gene
    RPTOR (a.k.a. KOG1, Mip1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer LKO.1 5'
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This plasmid is referred to as Raptor shRNA_2 in the science article. Sense: CCGGAGGGCC CTGCTACTCG CTTTTCTCGA GAAAAGCGAG TAGCAGGGCC CTTTTTTG. Antisense: AATTCAAAAA AGGGCCCTGC TACTCGCTTT TCTCGAGAAA AGCGAGTAGC AGGGCCC. For packaging, please use pCMV-dR8.2 dvpr (Addgene plasmid #8455) and pCMV-VSVG (Addgene plasmid #8454).

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Raptor_1 shRNA was a gift from David Sabatini (Addgene plasmid # 1857)
  • For your References section:

    Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Sarbassov DD, Guertin DA, Ali SM, Sabatini DM. Science 2005 Feb 18;307(5712):1098-101. 10.1126/science.1106148 PubMed 15718470