1
Plasmid 1857: Raptor_1 shRNA
  • Raptor

  • 65 Raptor_1

  • shRNA Raptor

  • 60

  • H. sapiens (human)

  • RPTOR (KOG1, Mip1)

  • pLKO.1
    (Search Vector Database)

  • Stewart SA, RNA 2003 Apr; 9(4):493-501.

  • Mammalian Expression, Lentiviral, RNAi

  • 7032

  • AgeI

  • Yes

  • EcoRI

  • No

  • LKO.1 5' List of Sequencing Primers

  • Ampicillin

  • Unknown

  • 37

  • XL10-Gold Ultracompetent Cells from Stratagene. 37oC.

  • Unknown

  • Puromycin

  • View sequences (3)
  • View map

  • View map

  • Addgene's pLKO.1 protocol

  • David Sabatini

  • UBMTA

Comments: 

This plasmid is referred to as Raptor shRNA_2 in the science article. Sense: CCGGAGGGCC CTGCTACTCG CTTTTCTCGA GAAAAGCGAG TAGCAGGGCC CTTTTTTG. Antisense: AATTCAAAAA AGGGCCCTGC TACTCGCTTT TCTCGAGAAA AGCGAGTAGC AGGGCCC. For packaging, please use pCMV-dR8.2 dvpr (Addgene plasmid #8455) and pCMV-VSVG (Addgene plasmid #8454).

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Sarbassov et al (Science 2005 Feb 18;307(5712):1098-101. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1857" in your Materials and Methods section.