Addgene - pWZL Blast DN E-cadherin Plasmid Data

Plasmid 18800: pWZL Blast DN E-cadherin

Gene/insert name:
Dom neg E-cadherin
Alt name:
E-Cadherin
Species:
M. musculus (mouse)
Entrez Gene:
Cdh1 (AA960649, E-cad, Ecad, L-CAM, UVO, Um)
Mutation:
truncated form of E-cadherin (dominant negative) lacking the ectodomain of the wild-type protein
Vector backbone:
pWZL-Blast
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5100
5' sequencing primer:
pBABE 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Blasticidin
Person or lab that originally
cloned the gene/insert:
E-cadherin cDNA provided by Dr. Gerhard Christofori
Sequence: View sequences (1)
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

Truncated murine E-cadherin cDNA cloned into pWZL blast.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Loss of E-cadherin promotes metastasis via multiple downstream transcriptional pathways. Onder et al (Cancer Res. 2008 May 15. 68(10):3645-54. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18800" in your Materials and Methods section.