Addgene - pLKO.1 hygro shRNA E-cadherin Plasmid Data

Plasmid 18802: pLKO.1 hygro shRNA E-cadherin

Gene/insert name:
E-cadherin shRNA
Alt name:
E-Cadherin
Species:
H. sapiens (human)
Entrez Gene:
CDH1 (Arc-1, CD324, CDHE, ECAD, LCAM, UVO)
Vector backbone:
pLKO.1 hygro
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, RNAi
5' sequencing primer:
LKO.1 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Hygromycin
Sequence: View sequences (1)
Supplemental document:
Addgene's pLKO.1 protocol
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

siRNA directed against E-cadherin: 5'-AAGATAGGAGTTCTCTGATGC-3'. Sequence from the website of the RNAi consortium at the Broad institute.

Please note that the sequence originally published in the paper was incorrect and the sequence above is the actual shRNA used.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Loss of E-cadherin promotes metastasis via multiple downstream transcriptional pathways. Onder et al (Cancer Res. 2008 May 15. 68(10):3645-54. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18802" in your Materials and Methods section.