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pLKO.1 puro shRNA E-cadherin
(Plasmid #18801)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 18801 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLKO.1 puro
  • Backbone manufacturer
    Available at Addgene (#8453)
  • Backbone size w/o insert (bp) 7000
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    E-cadherin shRNA
  • Alt name
    E-Cadherin
  • gRNA/shRNA sequence
    AAGATAGGAGTTCTCTGATGC
  • Species
    H. sapiens (human)
  • Entrez Gene
    CDH1 (a.k.a. Arc-1, BCDS1, CD324, CDHE, ECAD, LCAM, UVO)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (destroyed during cloning)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer LKO.1 5'
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

siRNA directed against E-cadherin: 5'-AAGATAGGAGTTCTCTGATGC-3'. Sequence from the website of the RNAi consortium at the Broad institute.

Please note that the sequence originally published in the paper was incorrect and the sequence above is the actual shRNA used.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLKO.1 puro shRNA E-cadherin was a gift from Bob Weinberg (Addgene plasmid # 18801 ; http://n2t.net/addgene:18801 ; RRID:Addgene_18801)
  • For your References section:

    Loss of E-cadherin promotes metastasis via multiple downstream transcriptional pathways. Onder TT, Gupta PB, Mani SA, Yang J, Lander ES, Weinberg RA. Cancer Res. 2008 May 15. 68(10):3645-54. 10.1158/0008-5472.CAN-07-2938 PubMed 18483246