pLKO.1 puro shRNA E-cadherin
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18801||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepLKO.1 puro
Backbone manufacturerAvailable at Addgene (#8453)
- Backbone size w/o insert (bp) 7000
Vector typeMammalian Expression, Lentiviral, RNAi
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameE-cadherin shRNA
SpeciesH. sapiens (human)
Entrez GeneCDH1 (a.k.a. Arc-1, BCDS1, CD324, CDHE, ECAD, LCAM, UVO)
- Promoter U6
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (destroyed during cloning)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer LKO.1 5' (Common Sequencing Primers)
siRNA directed against E-cadherin: 5'-AAGATAGGAGTTCTCTGATGC-3'. Sequence from the website of the RNAi consortium at the Broad institute.
Please note that the sequence originally published in the paper was incorrect and the sequence above is the actual shRNA used.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLKO.1 puro shRNA E-cadherin was a gift from Bob Weinberg (Addgene plasmid # 18801 ; http://n2t.net/addgene:18801 ; RRID:Addgene_18801)
For your References section:Loss of E-cadherin promotes metastasis via multiple downstream transcriptional pathways. Onder TT, Gupta PB, Mani SA, Yang J, Lander ES, Weinberg RA. Cancer Res. 2008 May 15. 68(10):3645-54. 10.1158/0008-5472.CAN-07-2938 PubMed 18483246