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tetO7_Ef1a_OCT4
(Plasmid #18918)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 18918 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Duet101
  • Backbone size w/o insert (bp) 10000
  • Vector type
    Mammalian Expression, Lentiviral, Cre/Lox
  • Selectable markers
    None

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    OCT4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1100
  • GenBank ID
    NM_002701
  • Entrez Gene
    POU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba1 (destroyed during cloning)
  • 3′ cloning site Cla1 (destroyed during cloning)
  • 5′ sequencing primer Ef1a
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    tetO7_Ef1a_OCT4 was a gift from Linzhao Cheng (Addgene plasmid # 18918)
  • For your References section:

    Improved efficiency and pace of generating induced pluripotent stem cells from human adult and fetal fibroblasts. Mali P, Ye Z, Hommond HH, Yu X, Lin J, Chen G, Zou J, Cheng L. Stem Cells. 2008 Aug . 26(8):1998-2005. 10.1634/stemcells.2008-0346 PubMed 18511599