Addgene - Ef1a_LIN28_Ires_Puro Plasmid Data

Plasmid 18921: Ef1a_LIN28_Ires_Puro

Gene/insert name:
LIN28
Insert size:
630
Species:
H. sapiens (human)
GenBank ID:
NM_024674
Entrez Gene:
LIN28A (CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A)
Vector backbone:
EGIP
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, Cre/Lox
Backbone size w/o insert (bp):
10000
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
Ef1a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Stbl3
High or low copy:
Unknown
Selectable markers:
Puromycin
Sequence: View sequences (1)
Principal Investigator:
Linzhao Cheng
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Improved Efficiency and Pace of Generating Induced Pluripotent Stem Cells from Human Adult and Fetal Fibroblasts. Mali et al (Stem Cells. 2008 May 29. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18921" in your Materials and Methods section.