Addgene - pDrBB2 Plasmid Data

Plasmid 18946: pDrBB2

Gene/insert name:
hygromycin gene driven by the copia promoter, phiC31 attB
Alt name:
phiC31 bacterial attachment site
Insert size:
3686
Species:
Escherichia coli
Vector backbone:
pHZ-attB
(Search Vector Database)
Vector type:
Insect Expression
Backbone size w/o insert (bp):
4622
Cloning site 5':
PvuII
Site destroyed during cloning:
Yes
Cloning site 3':
PflMI
Site destroyed during cloning:
Yes
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Hygromycin
Person or lab that originally
cloned the gene/insert:
hygromycin gene driven by the copia promoter from pMK33 (R. Nusse, Stanford University)
Sequence: View sequences (2)
Principal Investigator:
Michele Calos
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31. Groth et al (Genetics. 2004 Apr . 166(4):1775-82. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18946" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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