Addgene - p1xFlag CMV2 WW45 Plasmid Data

Plasmid 18970: p1xFlag CMV2 WW45

Gene/insert name:
SALVADOR 1
Alt name:
SAV1, SAV, WW45
Insert size:
1152
Species:
H. sapiens (human)
GenBank ID:
NM_021818
Entrez Gene:
SAV1 (SAV, WW45, WWP4)
Fusion protein or tag:
1xFlag
Terminal:
N terminal on backbone
Vector backbone:
p2xFlag-CMV2
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4700
Cloning site 5':
EcoRI
Site destroyed during cloning:
Unknown
Cloning site 3':
XhoI/SalI
Site destroyed during cloning:
Unknown
5' sequencing primer:
CMV List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
WW45 cDNA insert was derived from pME18SFL-WW45 construct obtained from Dr. Sumio Sugano, The Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minatoku, Tokyo, Japan.
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Marius Sudol
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Mst2 and Lats kinases regulate apoptotic function of YAP. Oka et al (J Biol Chem. 2008 Jul 17. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18970" in your Materials and Methods section.