p1xFlag CMV2 WW45
(Plasmid #18970)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    p2xFlag-CMV2
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    SALVADOR 1
  • Alt name
    SAV1, SAV, WW45
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1152
  • GenBank ID
    NM_021818
  • Entrez Gene
    SAV1 (a.k.a. SAV, WW45, WWP4)
  • Tag / Fusion Protein
    • 1xFlag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site XhoI/SalI (unknown if destroyed)
  • 5′ sequencing primer CMV
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    WW45 cDNA insert was derived from pME18SFL-WW45 construct obtained from Dr. Sumio Sugano, The Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minatoku, Tokyo, Japan.
  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p1xFlag CMV2 WW45 was a gift from Marius Sudol (Addgene plasmid # 18970)
  • For your References section:

    Mst2 and Lats kinases regulate apoptotic function of YAP. Oka T, Mazack V, Sudol M. J Biol Chem. 2008 Jul 17. ():. 10.1074/jbc.M804380200 PubMed 18640976