Addgene - pTrcHUM Plasmid Data

Plasmid 19003: pTrcHUM

Gene/insert name:
gamma-humulene synthase
Alt name:
HUM
Species:
A. grandis
Vector backbone:
pTrc99A
(Search Vector Database)
Backbone manufacturer:
pharmacia
Vector type:
Bacterial Expression
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Principal Investigator:
Jay Keasling
Terms and Licenses:
MTA

Comments:

gamma-humulene synthase has been codon optimized for E. coli

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Designed divergent evolution of enzyme function. Yoshikuni et al (Nature. 2006 Apr 20. 440(7087):1078-82. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19003" in your Materials and Methods section.