pBS-miR106a-363
(Plasmid #19028)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pBluescript KS(+)
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Mouse Targeting
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha
  • Copy number
    Unknown

Sequence Information

Gene/Insert

  • Gene/Insert name
    microRNA 106a-363
  • Alt name
    Mirn106a
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    11393
  • Entrez Gene
    Mir106a (a.k.a. Mirn106a)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site SmaI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-miR106a-363 was a gift from Tyler Jacks (Addgene plasmid # 19028)
  • For your References section:

    Targeted deletion reveals essential and overlapping functions of the miR-17 through 92 family of miRNA clusters. Ventura A, Young AG, Winslow MM, Lintault L, Meissner A, Erkeland SJ, Newman J, Bronson RT, Crowley D, Stone JR, Jaenisch R, Sharp PA, Jacks T. Cell. 2008 Mar 7. 132(5):875-86. 10.1016/j.cell.2008.02.019 PubMed 18329372