|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19028||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepBluescript KS(+)
- Backbone size w/o insert (bp) 3000
Vector typeMouse Targeting
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Gene/Insert namemicroRNA 106a-363
SpeciesM. musculus (mouse)
Insert Size (bp)11393
Entrez GeneMir106a (a.k.a. Mirn106a)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site SmaI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBS-miR106a-363 was a gift from Tyler Jacks (Addgene plasmid # 19028)
For your References section:Targeted deletion reveals essential and overlapping functions of the miR-17 through 92 family of miRNA clusters. Ventura A, Young AG, Winslow MM, Lintault L, Meissner A, Erkeland SJ, Newman J, Bronson RT, Crowley D, Stone JR, Jaenisch R, Sharp PA, Jacks T. Cell. 2008 Mar 7. 132(5):875-86. 10.1016/j.cell.2008.02.019 PubMed 18329372
Generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.