Addgene - pBS-miR106a-363 Plasmid Data

Plasmid 19028: pBS-miR106a-363

Gene/insert name:
microRNA 106a-363
Alt name:
Mirn106a
Insert size:
11393
Species:
M. musculus (mouse)
Entrez Gene:
Mir106a (Mirn106a)
Vector backbone:
pBluescript KS(+)
(Search Vector Database)
Backbone manufacturer:
Stratagene
Vector type:
Mouse Targeting
Backbone size w/o insert (bp):
3000
Cloning site 5':
KpnI
Site destroyed during cloning:
No
Cloning site 3':
SmaI
Site destroyed during cloning:
No
5' sequencing primer:
T3 List of Sequencing Primers
3' sequencing primer:
T7
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
DH5alpha
High or low copy:
Unknown
Selectable markers:
Neomycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Tyler Jacks
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Targeted deletion reveals essential and overlapping functions of the miR-17 through 92 family of miRNA clusters. Ventura et al (Cell. 2008 Mar 7. 132(5):875-86. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19028" in your Materials and Methods section.