PB-REVERBA-mScarlet-NLS-PEST
(Plasmid
#190658)
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PurposeFluorescent reporter for circadian rhythms
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 190658 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonePiggyBac Vector
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Backbone manufacturerSystem Biosciences
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameReverba-mScarlet
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SpeciesM. musculus (mouse)
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Entrez GeneNr1d1 (a.k.a. A530070C09Rik)
Resource Information
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Supplemental Documents
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: Plasmid contains multiple nucleotide discrepancies and a 219bp discrepancy in the RevErb Insert compared to the depositor's provided sequence. These discrepancies are not known to affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PB-REVERBA-mScarlet-NLS-PEST was a gift from Mary Teruel (Addgene plasmid # 190658 ; http://n2t.net/addgene:190658 ; RRID:Addgene_190658) -
For your References section:
The circadian clock mediates daily bursts of cell differentiation by periodically restricting cell-differentiation commitment. Zhang ZB, Sinha J, Bahrami-Nejad Z, Teruel MN. Proc Natl Acad Sci U S A. 2022 Aug 16;119(33):e2204470119. doi: 10.1073/pnas.2204470119. Epub 2022 Aug 8. 10.1073/pnas.2204470119 PubMed 35939672
Map uploaded by the depositor.
