Addgene - shp63alpha pLKO.1 puro Plasmid Data

Plasmid 19120: shp63alpha pLKO.1 puro

Gene/insert name:
small hairpin RNA against tumor protein p63
Alt name:
p63, AIS, B(p51A), B(p51B), EEC3, KET, LMS, NBP, OFC8, RHS, SHFM4, TP53CP, TP53L, TP73L, p40, p51, p53CP, p73H, p73L
Species:
H. sapiens (human)
Entrez Gene:
TP63 (AIS, B(p51A), B(p51B), EEC3, KET, LMS, NBP, OFC8, RHS, SHFM4, TP53CP, TP53L, TP73L, p40, p51, p53CP, p63, p73H, p73L)
Vector backbone:
pLKO.1 puro
(Search Vector Database)
Backbone manufacturer:
GenHunter
Vector type:
Mammalian Expression, Lentiviral, RNAi
Backbone size w/o insert (bp):
7032
5' sequencing primer:
LKO.1 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Use Stbl3 or HB101 to reduce chance of recombination.
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Map:
View map
Supplemental document:
Addgene's pLKO.1 protocol
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

shRNA against p63 (all isoforms), cloned by Watnick, RS.
shRNA sequence - 5'-CCG GCC GTT TCG TCA GAA CAC ACA TCT CGA GAT GTG TGT TCT GAC GAA ACG
GTT TTT

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Growth-inhibitory and tumor- suppressive functions of p53 depend on its repression of CD44 expression. Godar et al (Cell. 2008 Jul 11. 134(1):62-73. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19120" in your Materials and Methods section.