Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is open for ordering and depositing; find up-to-date details here. To learn more about how we are supporting COVID-19 research and to find related plasmids, check out our COVID-19 and Coronavirus Plasmids & Resources page.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

shp63alpha pLKO.1 puro
(Plasmid #19120)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 19120 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLKO.1 puro
  • Backbone manufacturer
    GenHunter
  • Backbone size w/o insert (bp) 7032
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Use Stbl3 or HB101 to reduce chance of recombination.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    small hairpin RNA against tumor protein p63
  • Alt name
    p63, AIS, B(p51A), B(p51B), EEC3, KET, LMS, NBP, OFC8, RHS, SHFM4, TP53CP, TP53L, TP73L, p40, p51, p53CP, p73H, p73L
  • Species
    H. sapiens (human)
  • Entrez Gene
    TP63 (a.k.a. AIS, B(p51A), B(p51B), EEC3, KET, LMS, NBP, OFC8, RHS, SHFM4, TP53CP, TP53L, TP73L, p40, p51, p53CP, p63, p73H, p73L)

Cloning Information

Resource Information

Depositor Comments

shRNA against p63 (all isoforms), cloned by Watnick, RS.
shRNA sequence - 5'-CCG GCC GTT TCG TCA GAA CAC ACA TCT CGA GAT GTG TGT TCT GAC GAA ACG
GTT TTT

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    shp63alpha pLKO.1 puro was a gift from Bob Weinberg (Addgene plasmid # 19120 ; http://n2t.net/addgene:19120 ; RRID:Addgene_19120)
  • For your References section:

    Growth-inhibitory and tumor- suppressive functions of p53 depend on its repression of CD44 expression. Godar S, Ince TA, Bell GW, Feldser D, Donaher JL, Bergh J, Liu A, Miu K, Watnick RS, Reinhardt F, McAllister SS, Jacks T, Weinberg RA. Cell. 2008 Jul 11. 134(1):62-73. 10.1016/j.cell.2008.06.006 PubMed 18614011