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deltaNp63alpha-FLAG
(Plasmid #26979)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26979 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1/hygro
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5597
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ΔNP63alpha
  • Alt name
    p63
  • Species
    H. sapiens (human)
  • Mutation
    dominant negative isoform of p63; aa 1–587
  • Entrez Gene
    TP63 (a.k.a. AIS, B(p51A), B(p51B), EEC3, KET, LMS, NBP, OFC8, RHS, SHFM4, TP53CP, TP53L, TP73L, p40, p51, p53CP, p63, p73H, p73L)
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

Addgene's quality control sequence shows an S59G mutation in the insert. The depositing lab does not believe this mutation affects function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    deltaNp63alpha-FLAG was a gift from David Sidransky (Addgene plasmid # 26979 ; http://n2t.net/addgene:26979 ; RRID:Addgene_26979)
  • For your References section:

    U-box-type ubiquitin E4 ligase, UFD2a attenuates cisplatin mediated degradation of DeltaNp63alpha. Chatterjee A, Upadhyay S, Chang X, Nagpal JK, Trink B, Sidransky D. Cell Cycle. 2008 May 1. 7(9):1231-7. 10.4161/cc.7.9.5795 PubMed 18418053