Addgene - shCD44-2 pRRL Plasmid Data

Plasmid 19123: shCD44-2 pRRL

Gene/insert name:
small hairpin RNA against CD44
Alt name:
CDW44, CSPG8, ECMR-III, HCELL, IN, LHR, MC56, MDU2, MDU3, MGC10468, MIC4, MUTCH-I, Pgp1
Insert size:
60
Species:
H. sapiens (human)
Entrez Gene:
CD44 (AL133330.1, CDW44, CSPG8, ECMR-III, HCELL, HUTCH-I, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1)
Vector backbone:
pRRL-U6-siluc-cppt-hPGKGFPSIN
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
7247
Cloning site 5':
AgeI
Site destroyed during cloning:
Unknown
Cloning site 3':
EcoRI
Site destroyed during cloning:
Unknown
5' sequencing primer:
EGFP N List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C, grows slowly.
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

shRNA to human CD44 (should target all isoforms). Subcloned via NdeI-BamHI frm pLKO1.Puro. This insert replaced identical sequence present in pRRL-EGFP and added the shRNA sequence

shRNA oligo sequence: sense:5'-CCGGTATGCAATGTGCTACTGATTGTCTCGAGACAATCAGTAGCACATTGCATTTTTTG-3'

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Growth-inhibitory and tumor- suppressive functions of p53 depend on its repression of CD44 expression. Godar et al (Cell. 2008 Jul 11. 134(1):62-73. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19123" in your Materials and Methods section.