Addgene - CD44P pGL3 Plasmid Data

Plasmid 19122: CD44P pGL3

Gene/insert name:
CD44 promoter
Alt name:
CDW44, CSPG8, ECMR-III, HCELL, IN, LHR, MC56, MDU2, MDU3, MGC10468, MIC4, MUTCH-I, Pgp1
Insert size:
2000
Species:
H. sapiens (human)
Entrez Gene:
CD44 (AL133330.1, CDW44, CSPG8, ECMR-III, HCELL, HUTCH-I, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1)
Vector backbone:
pGL3-Basic
(Search Vector Database)
Backbone manufacturer:
Promega
Vector type:
Non viral cloning vector
Backbone size w/o insert (bp):
4818
Cloning site 5':
NheI
Site destroyed during cloning:
Unknown
Cloning site 3':
XhoI
Site destroyed during cloning:
Unknown
5' sequencing primer:
RVprimer3 List of Sequencing Primers
3' sequencing primer:
LucNrev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Bob Weinberg
Terms and Licenses:
UBMTA
Luciferase Limited Use Label License

Comments:

PCR amplified from genomic DNA (human HMLE cells). Contains CD44 promoter, 2021bp upstream of translation initiation site.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Growth-inhibitory and tumor- suppressive functions of p53 depend on its repression of CD44 expression. Godar et al (Cell. 2008 Jul 11. 134(1):62-73. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19122" in your Materials and Methods section.