- Backbone size w/o insert (bp) 4818
Vector typeNon viral cloning vector
Growth in Bacteria
Copy numberLow Copy
Full plasmid sequence is available only if provided by the depositing laboratory.
Gene/Insert nameCD44 promoter
Alt nameCDW44, CSPG8, ECMR-III, HCELL, IN, LHR, MC56, MDU2, MDU3, MGC10468, MIC4, MUTCH-I, Pgp1
SpeciesH. sapiens (human)
Insert Size (bp)2000
Entrez GeneCD44 (a.k.a. CDW44, CSPG8, ECMR-III, HCELL, HUTCH-I, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1)
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer LucNrev (Common Sequencing Primers)
PCR amplified from genomic DNA (human HMLE cells). Contains CD44 promoter, 2021bp upstream of translation initiation site.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CD44P pGL3 was a gift from Bob Weinberg (Addgene plasmid # 19122)
For your References section:Growth-inhibitory and tumor- suppressive functions of p53 depend on its repression of CD44 expression. Godar S, Ince TA, Bell GW, Feldser D, Donaher JL, Bergh J, Liu A, Miu K, Watnick RS, Reinhardt F, McAllister SS, Jacks T, Weinberg RA. Cell. 2008 Jul 11. 134(1):62-73. 10.1016/j.cell.2008.06.006 PubMed 18614011
Map uploaded by the depositor.