Addgene - CD44S pWZL-Blast Plasmid Data

Plasmid 19126: CD44S pWZL-Blast

Gene/insert name:
CD44 standard
Alt name:
CDW44, CSPG8, ECMR-III, HCELL, IN, LHR, MC56, MDU2, MDU3, MGC10468, MIC4, MUTCH-I, Pgp1
Insert size:
1300
Species:
H. sapiens (human)
Entrez Gene:
CD44 (AL133330.1, CDW44, CSPG8, ECMR-III, HCELL, HUTCH-I, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1)
Vector backbone:
pWZL Blast
(Search Vector Database)
Backbone manufacturer:
Millenium Pharmaceuticals
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5100
Cloning site 5':
XhoI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
pBABE 5 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Use Stbl3 or HB101 to reduce chance of recombination.
High or low copy:
High Copy
Selectable markers:
Blasticidin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

Human CD44 cDNA,standard form no variable exons.
CD44 protein is stably expressed in MCF7Ras or HMEC-T cells but only transiently in BPEC-T cells. Use pBabe-CD44S for BPEC-T cells.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Growth-inhibitory and tumor- suppressive functions of p53 depend on its repression of CD44 expression. Godar et al (Cell. 2008 Jul 11. 134(1):62-73. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19126" in your Materials and Methods section.