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pBabepuro-myc-ER
(Plasmid #19128)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 19128 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBabepuro3:hbER tam
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    c-myc
  • Species
    H. sapiens (human)
  • Entrez Gene
    MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
  • Tag / Fusion Protein
    • mouse ER hormone binding domain (C terminal on insert)

Cloning Information

Resource Information

Depositor Comments

Please see Littlewood et al., Nucleic Acids Res. 1995 May 25;23(10):1686-90 for more information regarding the mutant hormone binding domain of the mouse estrogen receptor (hbER Tam).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabepuro-myc-ER was a gift from Wafik El-Deiry (Addgene plasmid # 19128 ; http://n2t.net/addgene:19128 ; RRID:Addgene_19128)
  • For your References section:

    Direct repression of FLIP expression by c-myc is a major determinant of TRAIL sensitivity. Ricci MS, Jin Z, Dews M, Yu D, Thomas-Tikhonenko A, Dicker DT, El-Deiry WS. Mol Cell Biol. 2004 Oct . 24(19):8541-55. 10.1128/MCB.24.19.8541-8555.2004 PubMed 15367674