pGL2-Mcl-1 mut
(Plasmid #19133)

• Depositing Lab: Wafik El-Deiry
• Publication: Ricci et al Cancer Cell. 2007 Jul . 12(1):66-80.

Backbone

• Vector backbone: pGL2-basic
• Backbone size w/o insert (bp): 5597
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Mcl-1 promoter
• Species: H. sapiens (human)
• Insert Size (bp): 325
• Mutation: NFkB binding-site mutant, the sequence GGGGTCTTCC in the NFkB-3 region was changed to GTTGTCTTCC.
• Entrez Gene: MCL1 (a.k.a. BCL2L3, EAT, MCL1-ES, MCL1L, MCL1S, Mcl-1, TM, bcl2-L-3, mcl1/EAT)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pGL2-Fwd

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry