Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19291||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5568
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)233
MutationE-cad promoter sequences from -108 to +125 Ebox elements were mutated from 5'-CANNTG-3' to 5'-AANNTA-3' (sense strand)
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer unknown
- 3′ sequencing primer luc-rev (Common Sequencing Primers)
Endogenous ATG of Ecad destroyed; "C" deleted 5 bp 5' of ATG (the destroyed one)
33 bp linker between Ecad promoter and luciferace gene.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGL2Basic-EcadK1/EpaIMUT/EboxMUT/Ebox2MUT was a gift from Eric Fearon (Addgene plasmid # 19291 ; http://n2t.net/addgene:19291 ; RRID:Addgene_19291)
For your References section:The SLUG zinc-finger protein represses E-cadherin in breast cancer. Hajra KM, Chen DY, Fearon ER. Cancer Res. 2002 Mar 15. 62(6):1613-8. PubMed 11912130
Map uploaded by the depositor.