|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19290||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5568
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)233
MutationE-cad promoter sequences from -108 to +125
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer unknown
- 3′ sequencing primer luc-rev (Common Sequencing Primers)
Endogenous ATG of Ecad destroyed; "C" deleted 5 bp 5' of ATG (the destroyed one)
33 bp linker between Ecad promoter and luciferace gene.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGL2Basic-EcadK1 was a gift from Eric Fearon (Addgene plasmid # 19290 ; http://n2t.net/addgene:19290 ; RRID:Addgene_19290)
For your References section:Extinction of E-cadherin expression in breast cancer via a dominant repression pathway acting on proximal promoter elements. Hajra KM, Ji X, Fearon ER. Oncogene. 1999 Dec 2. 18(51):7274-9. 10.1038/sj.onc.1203336 PubMed 10602481