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pGL2Basic-EcadK1
(Plasmid #19290)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 19290 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL2Basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5568
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    E-cadherin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    233
  • Mutation
    E-cad promoter sequences from -108 to +125

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer luc-rev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Endogenous ATG of Ecad destroyed; "C" deleted 5 bp 5' of ATG (the destroyed one)

33 bp linker between Ecad promoter and luciferace gene.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL2Basic-EcadK1 was a gift from Eric Fearon (Addgene plasmid # 19290 ; http://n2t.net/addgene:19290 ; RRID:Addgene_19290)
  • For your References section:

    Extinction of E-cadherin expression in breast cancer via a dominant repression pathway acting on proximal promoter elements. Hajra KM, Ji X, Fearon ER. Oncogene. 1999 Dec 2. 18(51):7274-9. 10.1038/sj.onc.1203336 PubMed 10602481