|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19133||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5597
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Gene/Insert nameMcl-1 promoter
SpeciesH. sapiens (human)
Insert Size (bp)325
MutationNFkB binding-site mutant, the sequence GGGGTCTTCC in the NFkB-3 region was changed to GTTGTCTTCC.
Entrez GeneMCL1 (a.k.a. BCL2L3, EAT, MCL1-ES, MCL1L, MCL1S, Mcl-1, TM, bcl2-L-3, mcl1/EAT)
- Cloning method Restriction Enzyme
- 5′ sequencing primer pGL2-Fwd (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGL2-Mcl-1 mut was a gift from Wafik El-Deiry (Addgene plasmid # 19133 ; http://n2t.net/addgene:19133 ; RRID:Addgene_19133)
For your References section:Reduction of TRAIL-induced Mcl-1 and cIAP2 by c-Myc or sorafenib sensitizes resistant human cancer cells to TRAIL-induced death. Ricci MS, Kim SH, Ogi K, Plastaras JP, Ling J, Wang W, Jin Z, Liu YY, Dicker DT, Chiao PJ, Flaherty KT, Smith CD, El-Deiry WS. Cancer Cell. 2007 Jul . 12(1):66-80. 10.1016/j.ccr.2007.05.006 PubMed 17613437