Single-cell quantitative expression reporters (scQers) Libraries
(Pooled Libraries #194097, #194098, #217420, #217421, #217422, #217423, #217424, #1000000239)
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Purpose
The plasmids are dual RNA reporter constructs (harboring two RNAs, each barcoded) for single-cell expression reporter assays, and contain pre-associated barcode pairs. Researchers can use these libraries by integrating their regulatory elements of interest. They then still need to perform another association (between enhancer and barcode), but the first laborious steps of the construction of reporter libraries are already done with these libraries.
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Vector Backbone
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Depositing Labs
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |||
|---|---|---|---|---|---|---|---|
| Pooled Library | 194097 | pre-associated oBC-mBC backbone (p025) | 1 | $ 1300 | Add to Cart | ||
| Pooled Library | 194098 | pre-associated oBC-minP-GFP-mBC backbone (p043) | 1 | $ 1300 | Add to Cart | ||
| Pooled Library | 217420 | scQers promoter control #1 (low: minimal promoter, p027) | 1 | $ 380 | Add to Cart | ||
| Pooled Library | 217421 | scQers promoter control #2 (low: no promoter, p029) | 1 | $ 380 | Add to Cart | ||
| Pooled Library | 217422 | scQers promoter control #3 (intermediate: UBC promoter, p041) | 1 | $ 380 | Add to Cart | ||
| Pooled Library | 217423 | scQers promoter control #4 (intermediate: Pgk1 promoter, p042) | 1 | $ 380 | Add to Cart | ||
| Pooled Library | 217424 | scQers promoter control #5 (high: EEF1A1 promoter, p028) | 1 | $ 380 | Add to Cart | ||
| Pooled Library | 1000000239 | scQers promoter control series (p027, p028, p029, p041, p042) | 1 | $ 1300 | Add to Cart | ||
Library Details
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Plasmids
p025 and p043: ~1,200,000 -
Control Plasmids
- p027: 250
- p029: 250
- p041: 250
- p042: 250
- p028: 250
Library Shipping
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.
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Volume∼20 µL
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Concentration20 ng/µL
Resource Information
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Protocols
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Terms and Licenses
Academic/Nonprofit Terms
Depositor Comments
The data and code (Link opens in a new window) related to these libraries is available on GitHub.
Please visit https://www.biorxiv.org/content/10.1101/2022.12.10.519236v1?rss=1 (Link opens in a new window) for bioRxiv preprint.
Figure 1. Schematic of cassette and workflow for single-cell quantitative expression reporters (scQers). Single-cell quantitative expression reporters (scQers) are dual barcoded RNA cassettes that separate the detection and quantification tasks, providing scalable and high-sensitivity readout of reporter expression in pooled single-cell contexts. Upon integration of libraries in multicellular system and single-cell profiling, the constitutively expressed detection barcode (blue, oBC) discriminates which cell received which reporter, and the matched quantification barcode (red, mBC) then enables unbiased measurement of the mRNA production from the associated cis-regulatory element.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Single-cell quantitative expression reporters (scQers) Libraries were a gift from Jay Shendure (Addgene #) -
For your References section:
Multiplex profiling of developmental enhancers with quantitative, single-cell expression reporters. Lalanne JB, Regalado SG, Domcke S, Calderon D, Martin B, Li T, Suiter CC, Lee C, Trapnell C, Shendure J. bioRxiv 2022.12.10.519236. doi: 10.1101/2022.12.10.519236.