Addgene - pAd-Oct4 Plasmid Data

Plasmid 19768: pAd-Oct4

Gene/insert name:
Oct4
Insert size:
2200
Species:
M. musculus (mouse)
Entrez Gene:
Pou5f1 (NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4)
Vector backbone:
pAd-ClaI
(Search Vector Database)
Vector type:
Mammalian Expression, Adenoviral
Backbone size w/o insert (bp):
32900
Cloning site 5':
MfeI
Site destroyed during cloning:
Unknown
Cloning site 3':
PacI
Site destroyed during cloning:
Unknown
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
Stbl3
High or low copy:
Low Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Konrad Hochedlinger
Terms and Licenses:
MTA

Comments:

From article: For the cloning of adenoviral vectors, EcoRI fragments containing the respective cDNAs
for c-myc (constitutively active human T58A variant), Klf4, Oct4 and Sox2 were
integrated into the EcoRI site of pHIHG-Ad2. A fragment containing the cDNA sequence
was isolated from the resulting plasmids by MfeI/PacI digestion and used for
electroporation of BJ5183 cells together with linearized vector pAd-ClaI. Correct clones
were identified by HindIII digestion.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Induced Pluripotent Stem Cells Generated Without Viral Integration. Stadtfeld et al (Science. 2008 Sep 25. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19768" in your Materials and Methods section.