- Backbone size w/o insert (bp) 32900
Vector typeMammalian Expression, Adenoviral
Growth in Bacteria
Copy numberLow Copy
Full plasmid sequence is available only if provided by the depositing laboratory.
SpeciesM. musculus (mouse)
Insert Size (bp)2200
Entrez GenePou5f1 (a.k.a. NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4)
- Cloning method Restriction Enzyme
- 5′ cloning site MfeI (unknown if destroyed)
- 3′ cloning site PacI (unknown if destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
From article: For the cloning of adenoviral vectors, EcoRI fragments containing the respective cDNAs
for c-myc (constitutively active human T58A variant), Klf4, Oct4 and Sox2 were
integrated into the EcoRI site of pHIHG-Ad2. A fragment containing the cDNA sequence
was isolated from the resulting plasmids by MfeI/PacI digestion and used for
electroporation of BJ5183 cells together with linearized vector pAd-ClaI. Correct clones
were identified by HindIII digestion.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAd-Oct4 was a gift from Konrad Hochedlinger (Addgene plasmid # 19768)
For your References section:Induced Pluripotent Stem Cells Generated Without Viral Integration. Stadtfeld M, Nagaya M, Utikal J, Weir G, Hochedlinger K. Science. 2008 Sep 25. ():. 10.1126/science.1162494 PubMed 18818365
Map uploaded by the depositor.