1
Plasmid 19768: pAd-Oct4
  • Oct4

  • 2200

  • M. musculus (mouse)

  • Pou5f1 (NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4)

  • pAd-ClaI
    (Search Vector Database)

  • Mammalian Expression, Adenoviral

  • 32900

  • MfeI

  • Unknown

  • PacI

  • Unknown

  • CMV-F List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • Stbl3

  • Low Copy

  • View sequences (1)
  • View map

  • Konrad Hochedlinger

  • UBMTA

Comments: 

From article: For the cloning of adenoviral vectors, EcoRI fragments containing the respective cDNAs
for c-myc (constitutively active human T58A variant), Klf4, Oct4 and Sox2 were
integrated into the EcoRI site of pHIHG-Ad2. A fragment containing the cDNA sequence
was isolated from the resulting plasmids by MfeI/PacI digestion and used for
electroporation of BJ5183 cells together with linearized vector pAd-ClaI. Correct clones
were identified by HindIII digestion.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Induced Pluripotent Stem Cells Generated Without Viral Integration. Stadtfeld et al (Science. 2008 Sep 25. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19768" in your Materials and Methods section.