Addgene - pCX-OKS-2A Plasmid Data

Plasmid 19771: pCX-OKS-2A

Gene/insert name:
Oct3/4-2A-Klf4-2A-Sox2
Alt name:
Pou5f1
Alt name:
Sox2
Alt name:
Klf4
Insert size:
3700
Species:
M. musculus (mouse)
Entrez Gene:
Sox2 (AL606746.1, Sox-2, lcc, ysb)
Klf4 (RP23-322L22.2, EZF, Gklf, Zie)
Pou5f1 (NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4)
Mutation:
Three genes are connected with the foot-and-mouth disease virus 2A self-cleaving sequence.
Vector backbone:
pCX
(Search Vector Database)
Backbone manufacturer:
Jun-ichi Miyazaki
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4778
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
GCGAGCCGCAGCCATTGCCTTTTA List of Sequencing Primers
3' sequencing primer:
TTAGCCAGAAGTCAGATGCTC
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
CAG Promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J.
Sequence: View sequences (5)
Map:
View map
Supplemental document:
Yamanaka gel (application/pdf)
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Shinya Yamanaka
Terms and Licenses:
MTA

Comments:

Addgene has verified the insert size with an EcoRI digest. To see a picture of the digest, click on "Reviews" under Plasmid Links, to the right of this page.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Generation of Mouse Induced Pluripotent Stem Cells Without Viral Vectors. Okita et al (Science. 2008 Oct 9. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19771" in your Materials and Methods section.