Addgene - pCX-cMyc Plasmid Data

Plasmid 19772: pCX-cMyc

Gene/insert name:
c-Myc
Insert size:
1350
Species:
M. musculus (mouse)
Entrez Gene:
Myc (AU016757, Myc2, Niard, Nird, bHLHe39)
Vector backbone:
pCX
(Search Vector Database)
Backbone manufacturer:
Jun-ichi Miyazaki
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4778
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
GCGAGCCGCAGCCATTGCCTTTTA List of Sequencing Primers
3' sequencing primer:
TTAGCCAGAAGTCAGATGCTC
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
CAG Promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J.
Sequence: View sequences (3)
Principal Investigator:
Shinya Yamanaka
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Generation of Mouse Induced Pluripotent Stem Cells Without Viral Vectors. Okita et al (Science. 2008 Oct 9. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 19772" in your Materials and Methods section.