pLX304_CMV::mCherry-LgBiT-FLAG-NES
(Plasmid #199715)

• Purpose: Expresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.
• Depositing Lab: Alice Ting
• Publication: Roh et al J Am Chem Soc. 2023 May 10;145(18):10220-10226. doi: 10.1021/jacs.3c01145. Epub 2023 Apr 26.

Backbone

• Vector backbone: pLX304
• Vector type: Mammalian Expression, Lentiviral, Luciferase
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry-LgBiT-FLAG-NES
• Species: Synthetic
• Promoter: CMV

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: WPRE-R

Resource Information

• Supplemental Documents:
  • mCherry-LgBiT-NES_07.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2023.03.03.531050 for bioRxiv preprint. In the original study XL1 Blue E. coli are used for amplifying plasmids.