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pMSP1
(Plasmid #20060)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 20060 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET 28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5300
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    BL-21(DE3); high oxygenation level
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MSP1
  • Alt name
    membrane scaffold protein;
  • Alt name
    apolipoprotein A-1
  • Alt name
    APOA1
  • Species
    synthetic gene
  • Insert Size (bp)
    640
  • Mutation
    synthetic gene of deletion mutant (1-43) of human Apolipoprotein A-1; contains N-terminal 6-His tag and Factor X site; the codons optimized for E. coli expression
  • Entrez Gene
    APOA1 (a.k.a. HPALP2, apo(a))
  • Tag / Fusion Protein
    • 6-His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nco I (not destroyed)
  • 3′ cloning site Hind III (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 term
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

First described in the following paper: Bayburt, T. H., Grinkova, Y. V., & Sligar, S. G., 2002, Self-assembly of discoidal phospholipid bilayer nanoparticles with membrane scaffold proteins. Nanoletters, 2: 853-856.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSP1 was a gift from Stephen Sligar (Addgene plasmid # 20060 ; http://n2t.net/addgene:20060 ; RRID:Addgene_20060)
  • For your References section:

    Directed self-assembly of monodisperse phospholipid bilayer Nanodiscs with controlled size. Denisov IG, Grinkova YV, Lazarides AA, Sligar SG. J Am Chem Soc. 2004 Mar 24. 126(11):3477-87. 10.1021/ja0393574 PubMed 15025475