Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20075||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5111
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1300
Entrez GeneMYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
/ Fusion Protein
- Flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pBABE 5 (Common Sequencing Primers)
Puro resistance cassette was removed by cutting pMSCVpuro with EcoRI and ClaI and religating. EcoRI site was maintained in the vector.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMSCV-Flag-cMyc T58A was a gift from Juan Belmonte (Addgene plasmid # 20075 ; http://n2t.net/addgene:20075 ; RRID:Addgene_20075)
For your References section:Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes. Aasen T, Raya A, Barrero MJ, Garreta E, Consiglio A, Gonzalez F, Vassena R, Bilić J, Pekarik V, Tiscornia G, Edel M, Boué S, Belmonte JC. Nat Biotechnol. 2008 Nov . 26(11):1276-84. 10.1038/nbt.1503 PubMed 18931654
Map uploaded by the depositor.