1
Plasmid 20075: pMSCV-Flag-cMyc T58A
  • cMyc

  • 1300

  • H. sapiens (human)

  • MYC (MRTL, MYCC, bHLHe39, c-Myc)

  • Flag

  • N terminal on insert

  • T58A

  • pMSCV
    (Search Vector Database)

  • Clontech

  • Mammalian Expression, Retroviral

  • 5111

  • XhoI

  • No

  • EcoRI

  • No

  • pBABE 5 List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (1)
  • View map

  • Juan Belmonte

  • UBMTA

Comments: 

Puro resistance cassette was removed by cutting pMSCVpuro with EcoRI and ClaI and religating. EcoRI site was maintained in the vector.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes. Aasen et al (Nat Biotechnol. 2008 Nov . 26(11):1276-84. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20075" in your Materials and Methods section.