Addgene - pMSCV-Flag-cMyc T58A Plasmid Data

Plasmid 20075: pMSCV-Flag-cMyc T58A

Gene/insert name:
cMyc
Insert size:
1300
Species:
H. sapiens (human)
Entrez Gene:
MYC (MRTL, bHLHe39, c-Myc)
Fusion protein or tag:
Flag
Terminal:
N terminal on insert
Mutation:
T58A
Vector backbone:
pMSCV
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5111
Cloning site 5':
XhoI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
pBABE 5 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Juan Belmonte
Terms and Licenses:
MTA

Comments:

Puro resistance cassette was removed by cutting pMSCVpuro with EcoRI and ClaI and religating. EcoRI site was maintained in the vector.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes. Aasen et al (Nat Biotechnol. 2008 Nov . 26(11):1276-84. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 20075" in your Materials and Methods section.