pPM28 (eroGFP CEN/ARS URA3)
(Plasmid #20131)

• Depositing Lab: Feroz Papa
• Publication: Merksamer et al Cell. 2008 Nov 28. 135(5):933-47.

Backbone

• Vector backbone: pRS316
• Backbone size w/o insert (bp): 4835
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: eroGFP
• Alt name: kar2ss-roGFP2-HDEL
• Alt name: KAR2
• Species: S. cerevisiae (budding yeast); jellyfish
• Insert Size (bp): 1828
• Entrez Gene: KAR2 (a.k.a. YJL034W, GRP78)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacII (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: S. James Remington University of Oregon
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry