pAAV-synapsin-WHaloCaMP1a
(Plasmid
#205308)
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PurposeProduction of AAV particles encoding WHaloCaMP1a from the synapsin promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 205308 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameWHaloCaMP1a
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.07.18.549527 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-synapsin-WHaloCaMP1a was a gift from Eric Schreiter (Addgene plasmid # 205308 ; http://n2t.net/addgene:205308 ; RRID:Addgene_205308) -
For your References section:
A modular chemigenetic calcium indicator enables in vivo functional imaging with near-infrared light. Farrants H, Shuai Y, Lemon WC, Hernandez CM, Yang S, Patel R, Qiao G, Frei MS, Grimm JB, Hanson TL, Tomaska F, Turner GC, Stringer C, Keller PJ, Beyene AG, Chen Y, Liang Y, Lavis LD, Schreiter ER. bioRxiv. 2023 Jul 19:2023.07.18.549527. doi: 10.1101/2023.07.18.549527. Preprint. 10.1101/2023.07.18.549527 PubMed 37503182