|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20894||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4800
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert name-290 Mix.2 M-p53BE promoter luciferase
SpeciesX. laevis (frog)
Insert Size (bp)290
Mutationpoint mutation of the p53 binding element (p53BE). Bears an additional EcoRI restriction site across the mutated sequence.
- Cloning method Restriction Enzyme
- 5′ cloning site SmaI (destroyed during cloning)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Mix.2-lux M-p53BE was a gift from Stefano Piccolo (Addgene plasmid # 20894 ; http://n2t.net/addgene:20894 ; RRID:Addgene_20894)
For your References section:Links between tumor suppressors: p53 is required for TGF-beta gene responses by cooperating with Smads. Cordenonsi M, Dupont S, Maretto S, Insinga A, Imbriano C, Piccolo S. Cell. 2003 May 2. 113(3):301-14. 10.1016/S0092-8674(03)00308-8 PubMed 12732139
Map uploaded by the depositor.