PB-TET-MKOS
(Plasmid #20959)

• Purpose: piggyBac vector with tet inducible cMyc, KLF4, Oct4, Sox2 for creation of induced pluripotent stem cells
• Depositing Lab: Andras Nagy
• Publication: Woltjen et al Nature. 2009 Mar 1. ():.

Backbone

• Vector backbone: PB-TET
• Backbone size w/o insert (bp): 9186
• Vector type: Mammalian Expression ; piggyBac
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cMyc, KLF4, Oct4, Sox2
• Species: M. musculus (mouse)
• Insert Size (bp): 5046
• Entrez Gene: Myc (a.k.a. Myc2, Niard, Nird, bHLHe39)
• Entrez Gene: Klf4 (a.k.a. EZF, Gklf, Zie)
• Entrez Gene: Pou5f1 (a.k.a. NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4)
• Entrez Gene: Sox2 (a.k.a. Sox-2, lcc, ysb)
• Tag / Fusion Protein:
  • IRES-bGeo (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: attB1 (not destroyed)
• 3′ cloning site: attB2 (not destroyed)
• 5′ sequencing primer: M13 F, T7
• 3′ sequencing primer: M13 R, T3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: MKOS liked by 2A peptides obtained from Keisuke Kaji et al (Nature. 2009 Mar 1) piggyBac terminal repeats obtained from Pentao Liu at Sanger.
• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Additional reference, Kaji et al Nature. 2009 Mar 1