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pmRFP_a_tubulin_IRES_puro2b
(Plasmid #21041)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21041 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    IRES_puro2b
  • Backbone size w/o insert (bp) 5776
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    alpha Tubulin
  • Alt name
    tubulin, alpha 1b
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1360
  • Entrez Gene
    TUBA1B (a.k.a. K-ALPHA-1)
  • Promoter CMV
  • Tag / Fusion Protein
    • pmRFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer IRES-R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmRFP_a_tubulin_IRES_puro2b was a gift from Daniel Gerlich (Addgene plasmid # 21041 ; http://n2t.net/addgene:21041 ; RRID:Addgene_21041)
  • For your References section:

    Aurora B-mediated abscission checkpoint protects against tetraploidization. Steigemann P, Wurzenberger C, Schmitz MH, Held M, Guizetti J, Maar S, Gerlich DW. Cell. 2009 Feb 6. 136(3):473-84. 10.1016/j.cell.2008.12.020 PubMed 19203582