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pGL3con-miR143Per
(Plasmid #21118)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21118 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3-control
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5256
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hsa-miR-143 sensor
  • Species
    Sequences containing two perfect complementary sites for hsa-miR-143
  • Insert Size (bp)
    56

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (destroyed during cloning)
  • 3′ cloning site XbaI (destroyed during cloning)
  • 5′ sequencing primer GTACCGAAAGGTCTTACCG
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3con-miR143Per was a gift from Joshua Mendell (Addgene plasmid # 21118 ; http://n2t.net/addgene:21118 ; RRID:Addgene_21118)
  • For your References section:

    Cell-cell contact globally activates microRNA biogenesis. Hwang HW, Wentzel EA, Mendell JT. Proc Natl Acad Sci U S A. 2009 Apr 9. ():. 10.1073/pnas.0811523106 PubMed 19359480